PDLIM7 Rekombinanter Antikörper

PDLIM7 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250775F12

Kat-Nr. : 86283-1-RR

Synonyme

ENIGMA, LIM mineralization protein, LMP1

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  WB analysis using 86283-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86283-1-RR (PDLIM7,LMP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of SKOV-3 using 86283-1-RR

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PDLIM7 antibody (86283-1-RR, Clone: 250775F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86283-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86283-1-RR against Human PDLIM7 were performed. The affinity constant is 8.36 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IF/ICC: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86283-1-RR bindet in WB, IF/ICC, ELISA PDLIM7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDLIM7 fusion protein Ag4614
• Vollständiger Name: PDZ and LIM domain 7 (enigma)
• Berechnetes Molekulargewicht: 55/60 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC001093
• Gene symbol: PDLIM7
• Gene ID (NCBI): 9260
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.