PDP2 Polyklonaler Antikörper

PDP2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 13404-1-AP

Synonyme

KIAA1348, PDP 2, PDP2, PDPC 2

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  WB analysis of mouse skeletal muscle using 13404-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13404-1-AP (PDP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13404-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13404-1-AP (PDP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13404-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13404-1-AP (PDP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13404-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13404-1-AP (PDP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 13404-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13404-1-AP (PDP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 13404-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13404-1-AP (PDP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 13404-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13404-1-AP (PDP2 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, HeLa-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13404-1-AP bindet in WB, IHC, ELISA PDP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDP2 fusion protein Ag4257
• Vollständiger Name: pyruvate dehydrogenase phosphatase isoenzyme 2
• Berechnetes Molekulargewicht: 529 aa, 60 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC028030
• Gene symbol: PDP2
• Gene ID (NCBI): 57546
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDP2, is the Ca2+-insensitive isoform of pyruvate dehydrogenase phosphataseis and found in liver and adipose tissue. In adipose tissue,insulin reduces the concentration dependence of PDP2 activity for Mg2+(PMID:12676647).It catalyzes the dephosphorylation and concomitant reactivation of the mitochondrial pyruvate dehydrogenase multienzyme complex.It can be detected the mature (52 kDa) and the immature (60 kDa) bands in western blot.

Publikationen

Species	Application	Title
mouse,human	WB	Dev Cell; Rheb mediates neuronal-activity-induced mitochondrial energetics through mTORC1-independent PDH activation.; Authors - Wanchun Yang
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma