PDRG1 Rekombinanter Antikörper

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• KD/KO Validated

PDRG1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250203E9

Kat-Nr. : 85851-4-RR

Synonyme

C20orf126, p53 and DNA damage regulated 1, p53 and DNA damage-regulated protein 1, PDRG

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  WB analysis using 85851-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85851-4-RR (PDRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 85851-4-RR

  WB result of PDRG1 antibody (85851-4-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDRG1 transfected Jurkat cells.

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  IHC staining of mouse cerebellum using 85851-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85851-4-RR (PDRG1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 85851-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85851-4-RR (PDRG1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85851-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85851-4-RR against Human PDRG1 were performed. The affinity constant is 0.653 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85851-4-RR bindet in WB, IHC, ELISA PDRG1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDRG1 fusion protein Ag9109
• Vollständiger Name: p53 and DNA damage regulated 1
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 15-16 kDa
• GenBank-Zugangsnummer: BC009334
• Gene symbol: PDRG1
• Gene ID (NCBI): 81572
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDRG1 codes for a novel protein called p53 and DNA damage-regulated protein 1. Human PDRG1 is predominantly expressed in normal testis and its expression can be induced by UV irradiation and be repressed by p53 [PMID14562055]. Recently, PDRG1 was identified as a novel tumor marker for multiple malignancies that is selectively regulated by genotoxic stress. Its expression was upregulated in multiple malignancies including cancers of the colon, rectum, ovary, lung, stomach, breast and uterus when compared to normal tissues [PMID: 21193842], suggesting its being a novel tumor marker that could play a role in cancer development and/or progression.