PDS5A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PDS5A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 17485-1-AP

Synonyme

SCC112, SCC 112, PIG54, PDS5, KIAA0648

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  WB analysis of HEK-293 using 17485-1-AP

  WB result of PDS5A antibody (17485-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDS5A transfected HEK-293 cells.

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  WB analysis using 17485-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17485-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17485-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 17485-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 17485-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17485-1-AP (PDS5A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 17485-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17485-1-AP (PDS5A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 17485-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17485-1-AP (PDS5A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 17485-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PDS5A antibody (17485-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

17485-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA PDS5A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDS5A fusion protein Ag11525
• Vollständiger Name: PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 1297 aa, 147 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC009650
• Gene symbol: PDS5A
• Gene ID (NCBI): 23244
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	EMBO J; Requirement of Nek2a and cyclin A2 for Wapl-dependent removal of cohesin from prophase chromatin; Authors - Susanne Hellmuth
human	WB	EMBO Rep; Regulation of cohesin-mediated chromosome folding by PDS5 in mammals; Authors - Dingdang Yu
mouse	WB,IF,CoIP	Sci Adv; The cohesin-associated protein Pds5A governs the meiotic spindle assembly via deubiquitination of Kif5B in oocytes; Authors - Yu Zhang ; KD Validated