PDS5A Polyklonaler Antikörper

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• KD/KO Validated

PDS5A Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17485-1-PBS

Synonyme

SCC112, SCC 112, PIG54, PDS5, KIAA0648

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  WB analysis using 17485-1-AP (same clone as 17485-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 17485-1-AP (same clone as 17485-1-PBS)

  WB result of PDS5A antibody (17485-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDS5A transfected HEK-293 cells. This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 17485-1-AP (same clone as 17485-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 17485-1-AP (same clone as 17485-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 17485-1-AP (same clone as 17485-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 17485-1-AP (same clone as 17485-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17485-1-AP (PDS5A Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 17485-1-AP (same clone as 17485-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17485-1-AP (PDS5A Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 17485-1-AP (same clone as 17485-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17485-1-AP (PDS5A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 17485-1-AP (same clone as 17485-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PDS5A antibody (17485-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17485-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PDS5A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDS5A fusion protein Ag11525
• Vollständiger Name: PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 1297 aa, 147 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC009650
• Gene symbol: PDS5A
• Gene ID (NCBI): 23244
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.