PDS5B Rekombinanter Antikörper

PDS5B Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250461B12

Kat-Nr. : 86088-2-RR

Synonyme

Androgen-induced proliferation inhibitor, Androgen-induced prostate proliferative shutoff-associated protein AS3, APRIN, AS3, CG008

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  WB analysis using 86088-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86088-2-RR (PDS5B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86088-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDS5B antibody (86088-2-RR, Clone: 250461B12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 86088-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDS5B antibody (86088-2-RR, Clone: 250461B12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86088-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86088-2-RR against Human PDS5B were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86088-2-RR bindet in WB, IF/ICC, ELISA PDS5B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDS5B fusion protein Ag27977
• Vollständiger Name: PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 165 aa
• Beobachtetes Molekulargewicht: 166 kDa
• GenBank-Zugangsnummer: NM_015032
• Gene symbol: PDS5B
• Gene ID (NCBI): 23047
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDS5B (PDS5 Cohesin Scc1-like Protein B) is a protein associated with sister chromatid cohesion and maintenance of chromosome structure. It plays a significant role in cell cycle regulation, DNA repair, and gene expression. PDS5B interacts with the cohesin complex to ensure the proper separation of sister chromatids during cell division. Additionally, PDS5B is crucial in DNA damage repair, particularly during homologous recombination, helping to maintain genomic stability. It also indirectly affects gene expression and chromatin structure by modulating the function of the cohesin complex. PDS5B is widely expressed during embryonic development and is highly expressed in the brain and testes in adulthood. Its dysfunction is associated with multiple diseases, including Cohesinopathies and Cornelia de Lange syndrome.