PDX1 Polyklonaler Antikörper

PDX1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 20989-1-AP

Synonyme

Glucose sensitive factor, GSF, IDX 1, IPF 1, IPF1, Islet/duodenum homeobox 1, IUF 1, IUF1, MODY4, PDX 1, PDX1, STF 1

---

Galerie der Validierungsdaten

• 

  WB analysis of BxPC-3 using 20989-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20989-1-AP (PDX1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20989-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20989-1-AP (PDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Produktinformation

20989-1-AP bindet in WB, IHC, IF, ELISA PDX1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: pancreatic and duodenal homeobox 1
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: NM_000209
• Gene symbol: PDX1
• Gene ID (NCBI): 3651
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pancreatic duodenal homeobox-1 protein (PDX-1), also designated INS promoter factor (IPF1), INS upstream factor 1 (IUF1), somatostatin trans-activating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic beta-cells. PDX-1 is a key regulator of pancreatic islet development and INS gene transcription in beta-cells. PDX-1 is expressed in all cells at the early stages of development and is mainly restricted to the pancreas and duodenum in adult. HNF-3b, HNF-1a and SP1 positively regulate the PDX-1 enhancer element. PDX-1 is also regulated by glucagon-like peptide through activation of adenylyl cyclase, which results in an increase in intracellular cAMP activity. The increased levels of cAMP, and the resulting activation of PKA, lead to an increase in PDX-1 transcription and translocation of the protein to the nuclei of beta-cells. PDX-1 binds to the sequence C(C/T) and can heterodimerize with PBX. PDX-1 is phosphorylated by the SAPK2 pathway under high glucose concentrations. Mutations in the PDX-1 gene can cause maturity-onset diabetes of the young and pancreatic agenesis.

Publikationen

Species	Application	Title
mouse	WB	ACS Appl Mater Interfaces; Targeted Polymeric Nanoparticles Based on Mangiferin for Enhanced Protection of Pancreatic β-Cells and Type 1 Diabetes Mellitus Efficacy.; Authors - Mengdi Wang
mouse	WB	Biomaterials; Three-dimensional cell-culture platform based on hydrogel with tunable microenvironmental properties to improve insulin-secreting function of MIN6 cells.; Authors - Miao Zhang
mouse	IF	Biofabrication; Dual-core coaxial bioprinting of double-channel constructs with a potential for perfusion and interaction of cells.; Authors - Yanrong Yu
mouse,rat	WB,IF	J Mol Cell Biol; Human umbilical cord-derived mesenchymal stem cells alleviate oxidative stress-induced islet impairment via the Nrf2/HO-1 axis; Authors - Peng Liu
mouse,rat	WB	J Biol Chem; Tectorigenin enhances PDX1 expression and protects pancreatic β-cells by activating ERK and reducing ER stress.; Authors - Xinlei Yao
mouse	WB	J Biol Chem; PMID: 29259128; Authors - Hang Li