PDXDC1 Polyklonaler Antikörper

PDXDC1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 21021-1-AP

Synonyme

Pyridoxal-dependent decarboxylase domain-containing protein 1, LP8165, KIAA0251, EC:4.1.1.-

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  WB analysis using 21021-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21021-1-AP (PDXDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21021-1-AP

  mouse testis lysates were subjected to SDS PAGE followed by western blot with 21021-1-AP (PDXDC1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 21021-1-AP

  IP result of anti-PDXDC1 (IP:21021-1-AP, 4ug; Detection:21021-1-AP 1:500) with HepG2 cells lysate 1680 ug.

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  IHC staining of human hepatocirrhosis using 21021-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21021-1-AP (PDXDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 21021-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21021-1-AP (PDXDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 21021-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21021-1-AP (PDXDC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 21021-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21021-1-AP (PDXDC1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 21021-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDXDC1 antibody (21021-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HeLa using 21021-1-AP

  Immunofluorescent analysis of Hela cells, using PDXDC1 antibody 21021-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushodengewebe, Rattenhodengewebe, SGC-7901-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

21021-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PDXDC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDXDC1 fusion protein Ag15283
• Vollständiger Name: pyridoxal-dependent decarboxylase domain containing 1
• Berechnetes Molekulargewicht: 788 aa, 87 kDa
• Beobachtetes Molekulargewicht: 87 kDa
• GenBank-Zugangsnummer: BC033748
• Gene symbol: PDXDC1
• Gene ID (NCBI): 23042
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDXDC1 (pyridoxal-dependent decarboxylase domain containing 1) is a putative enzyme that could metabolize catecholamine neurotransmitters (PMID:28485732). With prior evidence for involvement with glioblastoma from other previously reported experimental settings, and contains the lead single nucleotide polymorphism (rs3198697) from the linkage analysis of the chromosome 16 region (PMID:32644145). Moreover, PDXDC1 is involved in the catalysis of the nonhydrolytic addition or removal of a carboxyl group to or from a compound (PMID:29277971).

Publikationen

Species	Application	Title
mouse	IHC	J Mol Med (Berl); Identification of PDXDC1 as a novel pleiotropic susceptibility locus shared between lumbar spine bone mineral density and birth weight.; Authors - Yu-Qian Song
mouse	WB	Cell Mol Life Sci; Protease-independent control of parthanatos by HtrA2/Omi; Authors - Jonas Weiß
human	WB	Nat Commun; Thermal proteome profiling reveals fructose-1,6-bisphosphate as a phosphate donor to activate phosphoglycerate mutase 1; Authors - Yanling Zhang