PDZ-GEF2 Polyklonaler Antikörper

PDZ-GEF2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24583-1-AP

Synonyme

KIA001LB, PDZ GEF2, PDZGEF2, PDZ-GEF2, RA GEF 2, RAGEF2, RAPGEF6

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 24583-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 24583-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24583-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 24583-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 24583-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24583-1-AP bindet in WB, IHC, ELISA PDZ-GEF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDZ-GEF2 fusion protein Ag20325
• Vollständiger Name: Rap guanine nucleotide exchange factor (GEF) 6
• Berechnetes Molekulargewicht: 1601 aa, 179 kDa
• Beobachtetes Molekulargewicht: 179 kDa
• GenBank-Zugangsnummer: BC142964
• Gene symbol: RAPGEF6
• Gene ID (NCBI): 51735
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAPGEF6, also named PDZ-GEF2 enables several functions, including GTP-dependent protein binding activity; guanyl-nucleotide exchange factor activity; and phosphatidic acid binding activity. It is also involved in microvillus assembly; positive regulation of GTPase activity; and protein localization to plasma membrane. Located in several cellular components, including apical plasma membrane; centrosome; and endocytic vesicle.