PDZK1 Polyklonaler Antikörper

PDZK1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 10507-2-AP

Synonyme

CAP70, CFTR-associated protein of 70 kDa, Na(+)/H(+) exchange regulatory cofactor NHE-RF3, Na/Pi cotransporter C-terminal-associated protein 1, NaPi-Cap1

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  WB analysis of mouse kidney using 10507-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10507-2-AP (PDZK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10507-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10507-2-AP (PDZK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10507-2-AP

  IP result of anti-PDZK1 (IP:10507-2-AP, 3ug; Detection:10507-2-AP 1:500) with HEK-293 cells lysate 3200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

10507-2-AP bindet in WB, IHC, IF, IP, CoIP, ELISA PDZK1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDZK1 fusion protein Ag0787
• Vollständiger Name: PDZ domain containing 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 63-70 kDa
• GenBank-Zugangsnummer: BC006496
• Gene symbol: PDZK1
• Gene ID (NCBI): 5174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDZK1 is a PDZ domain-containing membrane scaffold protein widely expressed in a variety of epithelial cells. It plays a key role in cell signal transduction and ion channel regulation by interacting with a variety of ion channels, transporters, and receptors. PDZK1 simultaneously anchors multiple membrane proteins through its PDZ domain to regulate membrane expression and activity. PDZK1 maintains cholesterol homeostasis and participates in the regulation of the urate/anion exchanger (URAT1) in renal tubular uric acid reabsorption.

Publikationen

Species	Application	Title
human	WB	Oncotarget; Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.; Authors - Yi Huo
human	IHC	Mol Med; PDZK1 Is a Novel Factor in Breast Cancer That Is Indirectly Regulated by Estrogen through IGF-1R and Promotes Estrogen-Mediated Growth.; Authors - Kim Hogyoung H
human	IHC	Mol Med; Correlation between PDZK1, Cdc37, Akt and breast cancer malignancy: the role of PDZK1 in cell growth through Akt stabilization by increasing and interacting with Cdc37.; Authors - Hogyoung Kim
human	WB,IHC	Cell Div; miR-101-3p-mediated role of PDZK1 in hepatocellular carcinoma progression and the underlying PI3K/Akt signaling mechanism; Authors - Huihui Gao ; KD Validated
human	WB,IHC,CoIP	Aging (Albany NY); Promoter hypomethylated PDZK1 acts as a tumorigenic gene in glioma by interacting with AKT1; Authors - Xing Ren ; KD Validated
mouse,human	WB,IHC	Bone Res; PDZK1 protects against mechanical overload-induced chondrocyte senescence and osteoarthritis by targeting mitochondrial function; Authors - Yan Shao

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