PEA15 Rekombinanter Antikörper

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• KD/KO Validated

PEA15 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241044G8

Kat-Nr. : 83958-2-PBS

Synonyme

241044G8, Astrocytic phosphoprotein PEA-15, HMAT1, HUMMAT1H, MAT1

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  WB analysis using 83958-2-RR (same clone as 83958-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83958-2-RR (PEA15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83958-2-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 83958-2-RR (same clone as 83958-2-PBS)

  WB result of PEA15 antibody (83958-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEA15 transfected COLO 320 cells. This data was developed using the same antibody clone with 83958-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00916-1

  Cytometric bead array standard curve of MP00916-1, PEA15 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83958-2-PBS. Detection antibody: 83958-1-PBS. Standard: Ag13782. Range: 3.125-200 ng/mL.

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  Affinity and Kinetic Characterization of 83958-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83958-2-RR against Human PEA15 were performed. The affinity constant is 0.306 nM.

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Geprüfte Anwendungen

Produktinformation

83958-2-PBS bindet in WB, Cytometric bead array, Indirect ELISA PEA15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PEA15 fusion protein Ag13782
• Vollständiger Name: phosphoprotein enriched in astrocytes 15
• Berechnetes Molekulargewicht: 130 aa, 15 kDa
• Beobachtetes Molekulargewicht: 13-15 kDa
• GenBank-Zugangsnummer: BC002426
• Gene symbol: PEA15
• Gene ID (NCBI): 8682
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphoprotein Enriched in Astrocytes 15 kDa (PEA15), also known as PED-15, is a small, death effector-domain (DED)-containing protein that was recently demonstrated to inhibit tumor necrosis factor-α-induced apoptosis and to reverse the inhibition of integrin activation due to H-Ras. PEA15 is a 15 kDa protein that is highly expressed in the nervous system with particularly high levels in astrocytes and neurons of the hippocampus. PEA15 is a cytoplasmic protein that sits at an important junction in intracellular signalling and can regulate diverse cellular processes, such as proliferation and apoptosis, dependent upon stimulation.