PEA15 Rekombinanter Antikörper

PEA15 Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241044H6

Kat-Nr. : CL488-83958-3

Synonyme

MAT1H, MAT1, HUMMAT1H, HMAT1, Astrocytic phosphoprotein PEA-15

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  IF Staining of A431 using CL488-83958-3

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 PEA15 antibody (CL488-83958-3, Clone: 241044H6 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-83958-3 bindet in IF/ICC PEA15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PEA15 fusion protein Ag13782
• Vollständiger Name: phosphoprotein enriched in astrocytes 15
• Berechnetes Molekulargewicht: 130 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC002426
• Gene symbol: PEA15
• Gene ID (NCBI): 8682
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphoprotein Enriched in Astrocytes 15 kDa (PEA15), also known as PED-15, is a small, death effector-domain (DED)-containing protein that was recently demonstrated to inhibit tumor necrosis factor-α-induced apoptosis and to reverse the inhibition of integrin activation due to H-Ras. PEA15 is a 15 kDa protein that is highly expressed in the nervous system with particularly high levels in astrocytes and neurons of the hippocampus. PEA15 is a cytoplasmic protein that sits at an important junction in intracellular signalling and can regulate diverse cellular processes, such as proliferation and apoptosis, dependent upon stimulation.