RKIP Polyklonaler Antikörper

RKIP Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10575-1-AP

Synonyme

HCNP, HCNPpp, Neuropolypeptide h3, PBP, PEBP, PEBP 1, PEBP1, Prostatic binding protein, Raf kinase inhibitor protein, RKIP

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 10575-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10575-1-AP (RKIP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10575-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10575-1-AP (RKIP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10575-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10575-1-AP (RKIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 10575-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10575-1-AP (RKIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10575-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10575-1-AP (RKIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10575-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10575-1-AP (RKIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10575-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10575-1-AP (RKIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maushodengewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

10575-1-AP bindet in WB, IHC, ELISA RKIP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RKIP fusion protein Ag0864
• Vollständiger Name: phosphatidylethanolamine binding protein 1
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC008714
• Gene symbol: PEBP1
• Gene ID (NCBI): 5037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Raf kinase inhibitor protein (RKIP), also termed phosphatidylethanolamine binding protein PEBP1, was initially identified as a potent inhibitor of Raf-1/MEK/ERK, NF-kB, and G-protein-coupled receptor signaling pathways. Later RKIP has been further identified as a metastasis suppressor and its loss of expression has been reported in various cancers. Its expression has been proposed as a prognostic marker for patients diagnosed with the above cancers.

Publikationen

Species	Application	Title
human	IHC	FASEB J; Menstrual fluid factors facilitate tissue repair: identification and functional action in endometrial and skin repair.; Authors - Jemma Evans
human	IHC	Anticancer Res; Differential Protein Expression of Lymph Node Metastases of Papillary Thyroid Carcinoma Harboring the BRAF Mutation.; Authors - Park Won Seo WS
mouse	WB	J Ethnopharmacol; AR/RKIP pathway mediates the inhibitory effects of icariin on renal fibrosis and endothelial-to-mesenchymal transition in type 2 diabetic nephropathy; Authors - Wenhui Yao ; KD Validated