PEG3 Rekombinanter Antikörper

PEG3 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241234C5

Kat-Nr. : 84030-5-PBS

Synonyme

241234C5, KIAA0287, paternally expressed 3, Paternally-expressed gene 3 protein, PW1

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  WB analysis of TT using 84030-5-RR (same clone as 84030-5-PBS)

  TT cells were subjected to SDS PAGE followed by western blot with 84030-5-RR (PEG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84030-5-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 84030-5-RR (same clone as 84030-5-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 84030-5-RR (PEG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84030-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84030-5-RR (same clone as 84030-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84030-5-RR (PEG3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84030-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84030-5-RR (same clone as 84030-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84030-5-RR (PEG3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84030-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84030-5-RR (same clone as 84030-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PEG3 antibody (84030-5-RR, Clone: 241234C5 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84030-5-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84030-5-RR (same clone as 84030-5-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PEG3 Recombinant antibody (84030-5-RR, Clone:241234C5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84030-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84030-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84030-5-RR against Human PEG3 were performed. The affinity constant is 52.3 pM.

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Geprüfte Anwendungen

Produktinformation

84030-5-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA PEG3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PEG3 fusion protein Ag20132
• Vollständiger Name: paternally expressed 3
• Berechnetes Molekulargewicht: 1588 aa, 181 kDa
• Beobachtetes Molekulargewicht: 200-220 kDa
• GenBank-Zugangsnummer: BC150272
• Gene symbol: PEG3
• Gene ID (NCBI): 5178
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Paternally expressed 3 (PEG3) was responsible for Kras/Erk- and mTOR-driven HCC (hepatocellular carcinoma) (PMID: 36451866). PEG3 can regulate the physiological processes related to energy homeostasis found in multiple tissues, such as the brain, testis, ovary, and placenta (PMID: 32729618). Epigenetic silencing and loss of imprinting of PEG3 are implicated in glioma tumorigenesis (PMID: 32231463).