PELI1 Polyklonaler Antikörper

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• KD/KO Validated

PELI1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 12053-1-AP

Synonyme

PELI1, Pellino 1, pellino homolog 1 (Drosophila), PRISM, Protein pellino homolog 1

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  WB analysis of THP-1 using 12053-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 12053-1-AP (PELI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 12053-1-AP

  IP result of anti-PELI1 (IP:12053-1-AP, 4ug; Detection:12053-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

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  IHC staining of mouse brain using 12053-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12053-1-AP (PELI1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12053-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12053-1-AP (PELI1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

12053-1-AP bindet in WB, IP, IHC, ELISA PELI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PELI1 fusion protein Ag2683
• Vollständiger Name: pellino homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 418 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46-53 kDa
• GenBank-Zugangsnummer: BC011419
• Gene symbol: PELI1
• Gene ID (NCBI): 57162
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PELI1 encodes pellino-1, an E3 ubiquitin-protein ligase pellino homolog. E3 ubiquitin ligase functions by catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Pellino-1 is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Pellino-1 can mediate 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation thus playing a role in cellular inflammation. This antibody may cross react with Pellino-3.

Publikationen

Species	Application	Title
mouse	WB	PLoS Biol; Peli1 impairs microglial Aβ phagocytosis through promoting C/EBPβ degradation.; Authors - Jing Xu ; KO Validated
human,mouse	IHC	J Transl Med; MiR-30c-5p loss-induced PELI1 accumulation regulates cell proliferation and migration via activating PI3K/AKT pathway in papillary thyroid carcinoma.; Authors - Tingting Zheng
human	WB	Autoimmunity; MiR-153-3p induces immune dysregulation by inhibiting PELI1 expression in umbilical cord-derived mesenchymal stem cells in patients with systemic lupus erythematosus.; Authors - Dan Li
mouse	WB	Lab Invest; Regulatory T Cells Overexpressing Peli1 Show Better Efficacy in Repairing Ovarian Endocrine Function in Autoimmune Premature Ovarian Insufficiency; Authors - Yuan Wang
human	WB	Cell Signal; PELI1 overexpression contributes to pancreatic cancer progression through upregulating ubiquitination-mediated INPP5J degradation; Authors - Wenyang Zhou