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  • Featured Product
  • KD/KO Validated

PELI1 Polyklonaler Antikörper

PELI1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12053-1-AP

Synonyme

PELI1, Pellino 1, pellino homolog 1 (Drosophila), PRISM, Protein pellino homolog 1

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of THP-1 cells using PELI1 Polyclonal antibody (12053-1-AP)

    WB analysis of THP-1 using 12053-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 12053-1-AP (PELI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using PELI1 Polyclonal antibody (12053-1-AP)

    IP experiment of SH-SY5Y using 12053-1-AP

    IP Result of anti-PELI1 (IP:12053-1-AP, 4ug; Detection:12053-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PELI1 Polyclonal antibody (12053-1-AP)

    IHC staining of mouse brain using 12053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12053-1-AP (PELI1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PELI1 Polyclonal antibody (12053-1-AP)

    IHC staining of mouse brain using 12053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12053-1-AP (PELI1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBTHP-1-Zellen
Erfolgreiche IPSH-SY5Y-Zellen
Erfolgreiche Detektion in IHCMaushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

12053-1-AP bindet in WB, IP, IHC, ELISA PELI1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PELI1 fusion protein Ag2683
Vollständiger Name pellino homolog 1 (Drosophila)
Berechnetes Molekulargewicht 418 aa, 46 kDa
Beobachtetes Molekulargewicht 46-53 kDa
GenBank-ZugangsnummerBC011419
Gene symbol PELI1
Gene ID (NCBI) 57162
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PELI1 encodes pellino-1, an E3 ubiquitin-protein ligase pellino homolog. E3 ubiquitin ligase functions by catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Pellino-1 is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Pellino-1 can mediate 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation thus playing a role in cellular inflammation. This antibody may cross react with Pellino-3.

Protokolle

Produktspezifische Protokolle
WB protocol for PELI1 antibody 12053-1-APProtokoll herunterladen
IHC protocol for PELI1 antibody 12053-1-APProtokoll herunterladen
IP protocol for PELI1 antibody 12053-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

PLoS Biol

Peli1 impairs microglial Aβ phagocytosis through promoting C/EBPβ degradation.

Authors - Jing Xu
  • KO Validated
View Article
human,mouseIHC

J Transl Med

MiR-30c-5p loss-induced PELI1 accumulation regulates cell proliferation and migration via activating PI3K/AKT pathway in papillary thyroid carcinoma.

Authors - Tingting Zheng
View Article
humanWB

Autoimmunity

MiR-153-3p induces immune dysregulation by inhibiting PELI1 expression in umbilical cord-derived mesenchymal stem cells in patients with systemic lupus erythematosus.

Authors - Dan Li
View Article
mouseWB

Lab Invest

Regulatory T Cells Overexpressing Peli1 Show Better Efficacy in Repairing Ovarian Endocrine Function in Autoimmune Premature Ovarian Insufficiency

Authors - Yuan Wang
View Article