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PELI1 Polyklonaler Antikörper

PELI1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12053-1-PBS

Synonyme

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  • Western Blot (WB) analysis of THP-1 cells using PELI1 Polyclonal antibody (12053-1-AP)

    WB analysis of THP-1 using 12053-1-AP (same clone as 12053-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 12053-1-AP (PELI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12053-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using PELI1 Polyclonal antibody (12053-1-AP)

    IP experiment of SH-SY5Y using 12053-1-AP (same clone as 12053-1-PBS)

    IP result of anti-PELI1 (IP:12053-1-AP, 4ug; Detection:12053-1-AP 1:300) with SH-SY5Y cells lysate 3000ug. This data was developed using the same antibody clone with 12053-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PELI1 Polyclonal antibody (12053-1-AP)

    IHC staining of mouse brain using 12053-1-AP (same clone as 12053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12053-1-AP (PELI1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12053-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PELI1 Polyclonal antibody (12053-1-AP)

    IHC staining of mouse brain using 12053-1-AP (same clone as 12053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12053-1-AP (PELI1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12053-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12053-1-PBS bindet in WB, IHC, IP, Indirect ELISA PELI1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PELI1 fusion protein Ag2683
Vollständiger Name pellino homolog 1 (Drosophila)
Berechnetes Molekulargewicht 418 aa, 46 kDa
Beobachtetes Molekulargewicht 46-53 kDa
GenBank-ZugangsnummerBC011419
Gene symbol PELI1
Gene ID (NCBI) 57162
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PELI1 encodes pellino-1, an E3 ubiquitin-protein ligase pellino homolog. E3 ubiquitin ligase functions by catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Pellino-1 is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Pellino-1 can mediate 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation thus playing a role in cellular inflammation. This antibody may cross react with Pellino-3.