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PELI2 Polyklonaler Antikörper

PELI2 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16097-1-PBS

Synonyme

E3 ubiquitin-protein ligase pellino homolog 2, EC:2.3.2.27, Pellino 2, Pellino-2, Protein pellino homolog 2

Filter:
  • Western Blot (WB) analysis of various lysates using PELI2 Polyclonal antibody (16097-1-AP)

    WB analysis using 16097-1-AP (same clone as 16097-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16097-1-AP (PELI2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16097-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PELI2 Polyclonal antibody (16097-1-AP)

    IF Staining of HepG2 using 16097-1-AP (same clone as 16097-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PELI2 antibody (16097-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16097-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

16097-1-PBS bindet in WB, IF/ICC, Indirect ELISA PELI2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PELI2 fusion protein Ag8940
Vollständiger Name pellino homolog 2 (Drosophila)
Berechnetes Molekulargewicht 420 aa, 46 kDa
Beobachtetes Molekulargewicht 45-50 kDa
GenBank-ZugangsnummerBC009476
Gene symbol PELI2
Gene ID (NCBI) 57161
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pellino2 (PELI2) is a newly discovered E3 ubiquitin ligase, which regulates the protein degradation, protein-protein interaction, protein translocation and signaling transduction via the ubiquitination of target proteins, and plays important roles in inflammation and the immune system. PELI2 possesses a C-terminal RING-like domain and a phospho-threonine-binding forkhead-associated (FHA) domain that are responsible for ubiquitin ligase activity and substrate binding, respectively (PMID: 24699078).IHC