PELO Polyklonaler Antikörper

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• KD/KO Validated

PELO Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10582-1-PBS

Synonyme

CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog

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  WB analysis using 10582-1-AP (same clone as 10582-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 10582-1-AP (same clone as 10582-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10582-1-AP (same clone as 10582-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 10582-1-AP (same clone as 10582-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10582-1-AP (same clone as 10582-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10582-1-AP (same clone as 10582-1-PBS)

  IP result of anti-PELO (IP:10582-1-AP, 4ug; Detection:10582-1-AP 1:500) with HEK-293 cells lysate 1200ug. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 10582-1-AP (same clone as 10582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 10582-1-AP (same clone as 10582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 10582-1-AP (same clone as 10582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 10582-1-AP (same clone as 10582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IHC staining of rat ovary using 10582-1-AP (same clone as 10582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 10582-1-AP (same clone as 10582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10582-1-AP (same clone as 10582-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PELO antibody (10582-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10582-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PELO und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PELO fusion protein Ag0873
• Vollständiger Name: pelota homolog (Drosophila)
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43-45 kDa
• GenBank-Zugangsnummer: BC005889
• Gene symbol: PELO
• Gene ID (NCBI): 53918
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Pelo gene was originally identified in a mutagenesis screen for spermatogenesis-specific genes of Drosophila melanogaster. The PELO is required for the meiotic division during the G2/M transition, and functions in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs [PMID:12556505]. It may participate in the machinery of protein synthesis or in the regulation of mRNA translation [PMID:9584085].