PELO Rekombinanter Antikörper

PELO Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243137A4

Kat-Nr. : 85743-2-PBS

Synonyme

CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog

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  WB analysis using 85743-2-RR (same clone as 85743-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85743-2-RR (PELO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85743-2-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 85743-2-RR (same clone as 85743-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using PELO antibody (85743-2-RR, Clone: 243137A4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85743-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02111-2

  Cytometric bead array standard curve of MP02111-2, PELO Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85743-3-PBS. Detection antibody: 85743-2-PBS. Standard: Ag0873. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP02111-1

  Cytometric bead array standard curve of MP02111-1, PELO Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85743-1-PBS. Detection antibody: 85743-2-PBS. Standard: Ag0873. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 85743-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85743-2-RR against Human PELO were performed. The affinity constant is 0.152 nM.

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Geprüfte Anwendungen

Produktinformation

85743-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA PELO und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PELO fusion protein Ag0873
• Vollständiger Name: pelota homolog (Drosophila)
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43-45 kDa
• GenBank-Zugangsnummer: BC005889
• Gene symbol: PELO
• Gene ID (NCBI): 53918
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Pelo gene was originally identified in a mutagenesis screen for spermatogenesis-specific genes of Drosophila melanogaster. The PELO is required for the meiotic division during the G2/M transition, and functions in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs [PMID:12556505]. It may participate in the machinery of protein synthesis or in the regulation of mRNA translation [PMID:9584085].