PELP1 Monoklonaler Antikörper

PELP1 Monoklonal Antikörper für WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B11E4

Kat-Nr. : 67050-1-PBS

Synonyme

1B11E4, HMX3, MNAR, Modulator of non-genomic activity of estrogen receptor, Proline-, glutamic acid- and leucine-rich protein 1

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  WB analysis using 67050-1-Ig (same clone as 67050-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 67050-1-Ig (same clone as 67050-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67050-1-Ig (same clone as 67050-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67050-1-Ig (same clone as 67050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67050-1-Ig (same clone as 67050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 67050-1-Ig (same clone as 67050-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PELP1 (67050-1-Ig, Clone:1B11E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67050-1-PBS bindet in WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA PELP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PELP1 fusion protein Ag25729
• Vollständiger Name: proline, glutamate and leucine rich protein 1
• Berechnetes Molekulargewicht: 1130 aa, 120 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: BC069058
• Gene symbol: PELP1
• Gene ID (NCBI): 27043
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PELP1 was first identified as a 160 kDa protein in a screen for Src homology 2 (SH2) domain-binding proteins. PELP1 is overexpressed in 60-80% of breast tumors and plays important roles in both ER genomic and non-genomic signaling. In vivo, PELP1 subcellular localization is primarily nuclear in normal breast tissue, but it is localized to the cytoplasm in about 40% of invasive breast tumors. In the nucleus, PELP1 interacts with a number of transcription factors. The proto-oncogenic functions of PELP1 involve different cellular processes including epigenetic modifications leading to ER transactivation and breast cancer progression. Furthermore, PELP1 activates kinase cascades in the cytoplasm such as MAPK activation via c-Src and PI3K signaling.