PEMT Rekombinanter Antikörper

PEMT Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242150D5

Kat-Nr. : 85099-1-PBS

Synonyme

Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17

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  WB analysis of SH-SY5Y using 85099-1-RR (same clone as 85099-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85099-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85099-1-RR against Human PEMT were performed. The affinity constant is 0.379 nM.

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Geprüfte Anwendungen

Produktinformation

85099-1-PBS bindet in WB, IHC, Indirect ELISA PEMT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PEMT fusion protein Ag8034
• Vollständiger Name: phosphatidylethanolamine N-methyltransferase
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• GenBank-Zugangsnummer: BC050593
• Gene symbol: PEMT
• Gene ID (NCBI): 10400
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PEMT Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PMID:12431977, 15927961).