PEPD Polyklonaler Antikörper

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• KD/KO Validated

PEPD Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 12218-1-AP

Synonyme

EC:3.4.13.9, PRD, PROLIDASE, Xaa-Pro dipeptidase, X-Pro dipeptidase

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  WB analysis using 12218-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12218-1-AP

  WB result of PEPD antibody (12218-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEPD transfected HEK-293 cells.

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  WB analysis of MDA-MB-453s using 12218-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12218-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12218-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12218-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12218-1-AP

  WB result of PEPD antibody (12218-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEPD transfected HEK-293 cells.

• 

  WB analysis of HeLa using 12218-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12218-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 12218-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 12218-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12218-1-AP (PEPD antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A549 using 12218-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PEPD antibody (12218-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, MDA-MB-453s-Zellen, Maushautgewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:100-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

12218-1-AP bindet in WB, IHC, IF/ICC, ELISA PEPD und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PEPD fusion protein Ag2842
• Vollständiger Name: peptidase D
• Berechnetes Molekulargewicht: 493 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC015027
• Gene symbol: PEPD
• Gene ID (NCBI): 5184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)

Publikationen

Species	Application	Title
human	IHC	BMC Med Genet; Analysis of positional candidate genes in the AAA1 susceptibility locus for abdominal aortic aneurysms on chromosome 19.; Authors - Lillvis John H JH
human	IHC	Arch Med Res; Relationship Between Echocardiographically Evaluated Aortic Stiffness and Prolidase Activity in Aortic Tissue of Patients with Critical Coronary Artery Disease.; Authors - Aydemir Koçarslan
human	WB	Nat Commun; Parental experiences orchestrate locust egg hatching synchrony by regulating nuclear export of precursor miRNA; Authors - Ya Nan Zhu