PEPD Monoklonaler Antikörper

PEPD Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H2A1

Kat-Nr. : 67202-1-PBS

Synonyme

Imidodipeptidase, PEPD, peptidase D, PRD, PROLIDASE, Proline dipeptidase, X Pro dipeptidase, Xaa Pro dipeptidase

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  WB analysis using 67202-1-Ig (same clone as 67202-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 67202-1-Ig (same clone as 67202-1-PBS)

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67202-1-Ig (same clone as 67202-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67202-1-Ig (same clone as 67202-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67202-1-Ig (same clone as 67202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67202-1-Ig (PEPD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67202-1-Ig (same clone as 67202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67202-1-Ig (PEPD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67202-1-PBS bindet in WB, IHC, Indirect ELISA PEPD und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PEPD fusion protein Ag28713
• Vollständiger Name: peptidase D
• Berechnetes Molekulargewicht: 493 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC015027
• Gene symbol: PEPD
• Gene ID (NCBI): 5184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)