PER1 Polyklonaler Antikörper

PER1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13463-1-PBS

Synonyme

hPER, hPER1, KIAA0482, PER, period homolog 1 (Drosophila)

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  WB analysis of A549 using 13463-1-AP (same clone as 13463-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 13463-1-AP (same clone as 13463-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 13463-1-AP (same clone as 13463-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 13463-1-AP (same clone as 13463-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13463-1-AP (same clone as 13463-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13463-1-AP (same clone as 13463-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 13463-1-AP (same clone as 13463-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13463-1-AP (same clone as 13463-1-PBS)

  Immunofluorescent analysis of Hela cells, using PER1 antibody 13463-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13463-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PER1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PER1 fusion protein Ag4249
• Vollständiger Name: period homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 1290 aa, 136 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC028207
• Gene symbol: PER1
• Gene ID (NCBI): 5187
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PER1 is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation [PMID: 21514639]. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus [PMID: 9856465, 20930143]. In addition to bands of the predicted molecular mass of 140 kDa, additional PER1 species of 55 and 45 kDa is detected that is predominantly nuclear [PMID: 11726537] .