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PER1 Rekombinanter Antikörper

PER1 Rekombinant Antikörper für IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242096E3

Kat-Nr. : 84730-5-PBS

Synonyme

242096E3, hPER, hPER1, KIAA0482, PER

Filter:
  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PER1 Recombinant monoclonal antibody (84730-5-RR)

    IF Staining of MCF-7 using 84730-5-RR (same clone as 84730-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PER1 antibody (84730-5-RR, Clone: 242096E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84730-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using PER1 Recombinant monoclonal antibody (84730-5-RR)

    IF Staining of NIH/3T3 using 84730-5-RR (same clone as 84730-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PER1 antibody (84730-5-RR, Clone: 242096E3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84730-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using PER1 Recombinant monoclonal antibody (84730-5-RR)

    FC experiment of A549 using 84730-5-RR (same clone as 84730-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PER1 Recombinant antibody (84730-5-RR, Clone:242096E3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84730-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84730-5-RR

    Affinity and Kinetic Characterization of 84730-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84730-5-RR against Human PER1 were performed. The affinity constant is 0.802 nM.

Geprüfte Anwendungen

Produktinformation

84730-5-PBS bindet in IF/ICC, FC (Intra), Indirect ELISA PER1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PER1 fusion protein Ag4249
Vollständiger Name period homolog 1 (Drosophila)
Berechnetes Molekulargewicht 1290 aa, 136 kDa
GenBank-ZugangsnummerBC028207
Gene symbol PER1
Gene ID (NCBI) 5187
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PER1 is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation [PMID: 21514639]. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus [PMID: 9856465, 20930143].