PER1 Rekombinanter Antikörper

PER1 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242096E3

Kat-Nr. : 84730-5-RR

Synonyme

242096E3, hPER, hPER1, KIAA0482, PER

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  IF Staining of MCF-7 using 84730-5-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PER1 antibody (84730-5-RR, Clone: 242096E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of NIH/3T3 using 84730-5-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PER1 antibody (84730-5-RR, Clone: 242096E3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A549 using 84730-5-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug PER1 Recombinant antibody (84730-5-RR, Clone:242096E3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84730-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84730-5-RR against Human PER1 were performed. The affinity constant is 0.802 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84730-5-RR bindet in IF/ICC, FC (Intra), ELISA PER1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PER1 fusion protein Ag4249
• Vollständiger Name: period homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 1290 aa, 136 kDa
• GenBank-Zugangsnummer: BC028207
• Gene symbol: PER1
• Gene ID (NCBI): 5187
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PER1 is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation [PMID: 21514639]. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus [PMID: 9856465, 20930143].