PER2 Polyklonaler Antikörper

PER2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29954-1-AP

Synonyme

FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila)

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  WB analysis using 29954-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29954-1-AP (PER2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of MDA-MB-231 using 29954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using PER2 antibody (29954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: MDA-MB-231-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29954-1-AP bindet in WB, IF/ICC, ELISA PER2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PER2 fusion protein Ag31471
• Vollständiger Name: period homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 137KD
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: NM_022817
• Gene symbol: PER2
• Gene ID (NCBI): 8864
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774). 29954-1-AP detected molecular weight of 45 kDa.