PER2 (isoform 1) Monoklonaler Antikörper

PER2 (isoform 1) Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

CloneNo.

2E6C3

Rezensionen (1)

Kat-Nr. : 67513-1-Ig

Synonyme

PER2, PER 2, hPER2, FASPS, 2E6C3

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• 

  WB analysis using 67513-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67513-1-Ig (PER2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67513-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67513-1-Ig (PER2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 67513-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PER2 (isoform 1) antibody (67513-1-Ig, Clone: 2E6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, BxPC-3-Zellen, HL-60-Zellen, K-562-Zellen, L02-Zellen, THP-1-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

67513-1-Ig bindet in WB, IHC, IF/ICC, CoIP, ELISA PER2 (isoform 1) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PER2 (isoform 1) fusion protein Ag29489
• Vollständiger Name: period homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 137 kDa
• Beobachtetes Molekulargewicht: 150~160 kDa
• GenBank-Zugangsnummer: NM_022817
• Gene symbol: PER2
• Gene ID (NCBI): 8864
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, and 67513-1-Ig detected molecular weight of 150-160 kDa (PMID:16675517), while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774).

Publikationen

Species	Application	Title
	WB	Sci Total Environ; Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario; Authors - Fangnon Firmin Fangninou
human	WB	CNS Neurosci Ther; Cellular iron depletion enhances behavioral rhythm by limiting brain Per1 expression in mice; Authors - Qiong Wu
human	WB	J Biol Chem; M54 selectively stabilizes the circadian clock component of CRY1 and enhances the period of circadian rhythm at cellular level; Authors - Zeynep Melis Gül
human	WB	Cancer Manag Res; Circadian Clock Protein PERIOD2 Suppresses the PI3K/Akt Pathway and Promotes Cisplatin Sensitivity in Ovarian Cancer.; Authors - Zhaoxia Wang
human	WB	J Immunol Res; Suppression of DLBCL Progression by the E3 Ligase Trim35 Is Mediated by CLOCK Degradation and NK Cell Infiltration.; Authors - Xiyan Tan
human	WB,IHC	Front Mol Biosci; Period2 is associated with immune cell infiltration and is a potential diagnostic and prognostic marker for hepatocellular carcinoma; Authors - Xiaolin Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (01-05-2023)

I have been using this antibody for human keratinocytes, human cardiomyocytes and mouse tissues like liver and skin

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyoctyes, Keratinocytes, Liver and skin