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PER2 (isoform 1) Monoklonaler Antikörper

PER2 (isoform 1) Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E6C3

Kat-Nr. : 67513-1-PBS

Synonyme

PER2, PER 2, hPER2, FASPS, 2E6C3

Filter:
  • Western Blot (WB) analysis of various lysates using PER2 (isoform 1) Monoclonal antibody (67513-1-Ig)

    WB analysis using 67513-1-Ig (same clone as 67513-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67513-1-Ig (PER2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67513-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PER2 (isoform 1) Monoclonal antibody (67513-1-Ig)

    WB analysis using 67513-1-Ig (same clone as 67513-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67513-1-Ig (PER2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67513-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PER2 (isoform 1) Monoclonal antibody (67513-1-Ig)

    IF Staining of HeLa using 67513-1-Ig (same clone as 67513-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PER2 (isoform 1) antibody (67513-1-Ig, Clone: 2E6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67513-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67513-1-PBS bindet in WB, IF/ICC, Indirect ELISA PER2 (isoform 1) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen PER2 (isoform 1) fusion protein Ag29489
Vollständiger Name period homolog 2 (Drosophila)
Berechnetes Molekulargewicht 137 kDa
Beobachtetes Molekulargewicht150~160 kDa
GenBank-ZugangsnummerNM_022817
Gene symbol PER2
Gene ID (NCBI) 8864
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, and 67513-1-Ig detected molecular weight of 150-160 kDa (PMID:16675517), while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774).