PERK/EIF2AK3 Monoklonaler Antikörper

PERK/EIF2AK3 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

3G3F2

Kat-Nr. : 68482-1-Ig

Synonyme

EIF2AK3, PERK, 3G3F2, EC:2.7.11.1, EIF2AK 3

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  WB analysis using 68482-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68482-1-Ig (PERK/EIF2AK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68482-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68482-1-Ig (PERK/EIF2AK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, HEK-293-Zellen, HuH-7-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

68482-1-Ig bindet in WB, ELISA PERK/EIF2AK3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PERK/EIF2AK3 fusion protein Ag18254
• Vollständiger Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Berechnetes Molekulargewicht: 1116 aa, 125 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC126354
• Gene symbol: PERK
• Gene ID (NCBI): 9451
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).

Publikationen

Species	Application	Title
pig	WB	Int J Mol Sci; Evaluation of Cross-Talk and Alleviate Potential of Cytotoxic Factors Induced by Deoxynivalenol in IPEC-J2 Cells Interference with Curcumin; Authors - Qiyuan Wang
human	WB	Cancer Gene Ther; N6-methyladenosine modification of RIMS binding protein 2 promotes head and neck squamous cell carcinoma proliferation and radiotherapy tolerance through endoplasmic reticulum stress; Authors - Xinyu Sun
human	WB	FASEB J; Luteolin Modulate Endoplasmic Reticulum Stress by Targeting SIRT1 to Ameliorate DSS-Induced Ulcerative Colitis in Mice; Authors - Hai-Xiang Guo
human	WB	Free Radic Biol Med; Ultrasound-Responsive Taurine Lipid Nanoparticles Attenuate Oxidative Stress and Promote Macrophage Polarization for Diabetic Wound Healing; Authors - Zucheng Luo