• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PERK/EIF2AK3 Monoklonaler Antikörper

PERK/EIF2AK3 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G3F2

Kat-Nr. : 68482-1-PBS

Synonyme

EIF2AK3, PERK, 3G3F2, EC:2.7.11.1, EIF2AK 3

Filter:
  • Western Blot (WB) analysis of various lysates using PERK/EIF2AK3 Monoclonal antibody (68482-1-Ig)

    WB analysis using 68482-1-Ig (same clone as 68482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68482-1-Ig (PERK/EIF2AK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PERK/EIF2AK3 Monoclonal antibody (68482-1-Ig)

    WB analysis using 68482-1-Ig (same clone as 68482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68482-1-Ig (PERK/EIF2AK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68482-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68482-1-PBS bindet in WB, Indirect ELISA PERK/EIF2AK3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen PERK/EIF2AK3 fusion protein Ag18254
Vollständiger Name eukaryotic translation initiation factor 2-alpha kinase 3
Berechnetes Molekulargewicht 1116 aa, 125 kDa
Beobachtetes Molekulargewicht140 kDa
GenBank-ZugangsnummerBC126354
Gene symbol PERK
Gene ID (NCBI) 9451
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).