PES1 Polyklonaler Antikörper

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PES1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13553-1-PBS

Synonyme

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  WB analysis of COLO 320 using 13553-1-AP (same clone as 13553-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 13553-1-AP (same clone as 13553-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

• 

  IP experiment of COLO 320 using 13553-1-AP (same clone as 13553-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:300. This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 13553-1-AP (same clone as 13553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13553-1-AP (PES1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 13553-1-AP (same clone as 13553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13553-1-AP (PES1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 13553-1-AP (same clone as 13553-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13553-1-AP (PES1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13553-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PES1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PES1 fusion protein Ag4342
• Vollständiger Name: pescadillo homolog 1, containing BRCT domain (zebrafish)
• Berechnetes Molekulargewicht: 588 aa, 68 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC032489
• Gene symbol: PES1
• Gene ID (NCBI): 23481
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.