PEX12 Polyklonaler Antikörper

PEX12 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 27011-1-AP

Synonyme

PEX, Peroxisome assembly protein 12, Peroxisome assembly factor 3, Peroxin-12, Peroxin 12

---

• 

  WB analysis of MCF-7 using 27011-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 27011-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27011-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 27011-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27011-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

27011-1-AP bindet in WB, ELISA PEX12 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PEX12 fusion protein Ag25500
• Vollständiger Name: peroxisomal biogenesis factor 12
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 40-42 kDa
• GenBank-Zugangsnummer: BC031085
• Gene symbol: PEX12
• Gene ID (NCBI): 5193
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxisome assembly protein 12 (PEX12), also known as Peroxisome assembly factor 3 (PAF-3), is an integral peroxisomal membrane protein that is essential for peroxisomal matrix protein import and is one of only two peroxins known to interact with the ligand-binding domain of PEX5. Mutations in human PEX12 result in Zellweger syndrome, a lethal neurological disorder, and implicate the zinc ring domain in PEX12 function (PMID: 12456682). Pex12 exhibits protein-Ub ligase activity with specificity for the E2 protein Pex4 and Ubc4 (PMID: 19687296).

Publikationen

Species	Application	Title
mouse	WB	Commun Biol; PEX3 promotes regenerative repair after myocardial injury in mice through facilitating plasma membrane localization of ITGB3; Authors - Jia-Teng Sun
pig	WB	PLoS Pathog; SIRT5-mediated desuccinylation of the porcine deltacoronavirus M protein drives pexophagy to enhance viral proliferation; Authors - Zhuang Li