PEX16 Polyklonaler Antikörper

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• KD/KO Validated

PEX16 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 14816-1-AP

Synonyme

Peroxin 16, PEX16

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Galerie der Validierungsdaten

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  WB analysis of human liver using 14816-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14816-1-AP (PEX16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 14816-1-AP

  Immunofluorescent analysis of HepG2 cells using 14816-1-AP (PEX16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14816-1-AP bindet in WB, IHC, IF, ELISA PEX16 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, arabidopsis, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PEX16 fusion protein Ag6566
• Vollständiger Name: peroxisomal biogenesis factor 16
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC000467
• Gene symbol: PEX16
• Gene ID (NCBI): 9409
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 16, also known as PEX16 or Peroxisomal biogenesis factor 16, is a 336 amino acid integral membrane protein that has a critical role in the biogenesis of peroxisomes. PEX16 together with PEX3 and PEX19 are specifically involved in peroxisomal membrane protein (PMP) import. Defects in the gene encoding Peroxin 16 are the cause of multiple peroxisome-related disorders, including Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), classical rhizomelic chondrodysplasia punctata (RCDP) and peroxisome biogenesis disorder complementation group 9 (PBD-CG9).

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.; Authors - Katharina M Walter
mouse	WB,IF	J Clin Invest; Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission.; Authors - Hongsuk Park ; KO Validated
mouse	WB	Nat Commun; Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling.; Authors - Celia de la Calle Arregui
human	WB	Cell Death Differ; Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis.; Authors - Weiwei Cui
human	WB	Proc Natl Acad Sci U S A; Sec16B is involved in the endoplasmic reticulum export of the peroxisomal membrane biogenesis factor peroxin 16 (Pex16) in mammalian cells.; Authors - Yonekawa Shusuke S
human	WB	Cell Death Differ; Peroxisomes protect lymphoma cells from HDAC inhibitor-mediated apoptosis.; Authors - Michael S Dahabieh