PEX19 Monoklonaler Antikörper

PEX19 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3H2C2

Kat-Nr. : 68555-1-PBS

Synonyme

PMPI, PMP1, PEX, Peroxin-19, OK/SW-cl.22

---

• 

  WB analysis using 68555-1-Ig (same clone as 68555-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68555-1-Ig (PEX19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68555-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68555-1-Ig (same clone as 68555-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PEX19 antibody (68555-1-Ig, Clone: 3H2C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68555-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 68555-1-Ig (same clone as 68555-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PEX19 antibody (68555-1-Ig, Clone: 3H2C2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68555-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

68555-1-PBS bindet in WB, IF/ICC, Indirect ELISA PEX19 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PEX19 fusion protein Ag6858
• Vollständiger Name: peroxisomal biogenesis factor 19
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC000496
• Gene symbol: PEX19
• Gene ID (NCBI): 5824
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. PEX19 gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs) (PMID: 14709540). PEX19 may bind newly synthesized PMPs and facilitate their insertion into the peroxisome membrane (PMID: 107044440. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. Defects in this gene are a cause of Zellweger syndrome (ZWS), as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14) (PMID:20683989) (PMID:10051604).