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PEX5 Monoklonaler Antikörper

PEX5 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C2C3

Kat-Nr. : 66309-1-Ig

Synonyme

Peroxin 5, Peroxisome Marker, Peroxisome receptor 1, PEX5, PTS1 BP, PTS1 receptor, PTS1R, PXR1

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of HEK-293 cells using PEX5 Monoclonal antibody (66309-1-Ig)

    WB analysis of HEK-293 using 66309-1-Ig

    WB result of PEX5 antibody (66309-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX5 transfected HEK-293 cells.

  • Western Blot (WB) analysis of pig brain tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    WB analysis of pig brain using 66309-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66309-1-Ig (PEX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    WB analysis of fetal human brain using 66309-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66309-1-Ig (PEX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human liver using 66309-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human liver using 66309-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human kidney using 66309-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human kidney using 66309-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 40x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHausschwein-Hirngewebe, fetales humanes Hirngewebe, HEK-293-Zellen
Erfolgreiche Detektion in IHChumanes Lebergewebe, humanes Nierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

66309-1-Ig bindet in WB, IHC, ELISA PEX5 und zeigt Reaktivität mit Hausschwein, human, Ratten

Getestete Reaktivität Hausschwein, human, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen PEX5 fusion protein Ag3268
Vollständiger Name peroxisomal biogenesis factor 5
Berechnetes Molekulargewicht 631 aa, 70 kDa
Beobachtetes Molekulargewicht 70-80 kDa
GenBank-ZugangsnummerBC010621
Gene symbol PEX5
Gene ID (NCBI) 5830
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The peroxisomal targeting signal type1 (PTS1) receptor, PEX5, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively, and PEX5 binds PTS1 through its C-terminal 40-kDa tetratricopeptide repeat domain. It is a predominantly cytoplasmic, partly peroxisomal protein that appears to shuttle between these compartments as it mediates the import of PTS1-containing proteins. PEX5 has been reported to interact with PEX10, PEX12, PEX13, and PEX14. Defects in PEX5 are a cause of Zellweger syndrome (ZWS), which is a lethal peroxisome biogenesis disorder.

Protokolle

Produktspezifische Protokolle
WB protocol for PEX5 antibody 66309-1-IgProtokoll herunterladen
IHC protocol for PEX5 antibody 66309-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

EMBO J

BNIP3L/NIX regulates both mitophagy and pexophagy

Authors - Léa P Wilhelm
View Article