CXCL4/PF4 Polyklonaler Antikörper

CXCL4/PF4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 21157-1-AP

Synonyme

CXCL4, PF4, SCYB4, C X C motif chemokine 4, C-X-C motif chemokine 4

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  WB analysis of human peripheral blood platelets using 21157-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 21157-1-AP (PF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21157-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21157-1-AP (PF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse serum using 21157-1-AP

  IP result of anti-PF4 (IP:21157-1-AP, 4ug; Detection:21157-1-AP 1:200) with mouse serum lysate 1925 ug.

• 

  IHC staining of mouse spleen using 21157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21157-1-AP (PF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21157-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PF4 antibody (21157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humane Thrombozyten aus peripherem Blut, Mausmilzgewebe
• Erfolgreiche IP: mouse serum
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

21157-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CXCL4/PF4 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCL4/PF4 fusion protein Ag12345
• Vollständiger Name: platelet factor 4
• Berechnetes Molekulargewicht: 101 aa, 11 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC093965
• Gene symbol: PF4
• Gene ID (NCBI): 5196
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The chemokine (C-X-C motif) ligand 4 (CXCL4) is also named as PF4 (Platelet factor-4) and SCYB4. The platelets, the most abundant source of CXCL4 in vivo, control profibrotic macrophage activation via CXCL4. (PMID: 36807143). Exogenous CXCL4 drove profibrotic activation of monocyte-derived dendritic cells in vitro (PMID: 33042127). Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation.

Publikationen

Species	Application	Title
mouse	WB,IHC	Int Immunopharmacol; CXCL4:NLRP3-mediated pyroptosis product that regulates cardiac fibrosis; Authors - Jing Wei
human,mouse	WB,IHC	J Virol; Chemokine PF4 Inhibits EV71 and CA16 Infections at the Entry Stage.; Authors - Zhichao Pei
human,mouse	WB	Toxicol Appl Pharmacol; Synergistic anticancer effects of ginsenoside CK and gefitinib against gefitinib-resistant NSCLC by regulating the balance of angiogenic factors through HIF-1α/VEGF; Authors - Xiaoping Song
human	WB	J Virol; Platelet factor 4-derived C15 peptide broadly inhibits enteroviruses by disrupting viral attachment; Authors - Shuai Lv
human,mouse	IF	Int J Mol Med; Platelet activation stimulates macrophages to enhance ulcerative colitis through PF4/CXCR3 signaling; Authors - Yuxiao Niu
mouse	WB	J Biol Chem; ELF4 facilitates innate host defenses against Plasmodium by activating transcription of Pf4 and Ppbp; Authors - Dandan Wang