CXCL4/PF4 Polyklonaler Antikörper

CXCL4/PF4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21157-1-PBS

Synonyme

CXCL4, PF4, SCYB4, C X C motif chemokine 4, C-X-C motif chemokine 4

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  WB analysis of human peripheral blood platelets using 21157-1-AP (same clone as 21157-1-PBS)

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 21157-1-AP (PF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21157-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse spleen using 21157-1-AP (same clone as 21157-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21157-1-AP (PF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21157-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse serum using 21157-1-AP (same clone as 21157-1-PBS)

  IP result of anti-PF4 (IP:21157-1-AP, 4ug; Detection:21157-1-AP 1:200) with mouse serum lysate 1925 ug. This data was developed using the same antibody clone with 21157-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 21157-1-AP (same clone as 21157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21157-1-AP (PF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21157-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 21157-1-AP (same clone as 21157-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PF4 antibody (21157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21157-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21157-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CXCL4/PF4 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCL4/PF4 fusion protein Ag12345
• Vollständiger Name: platelet factor 4
• Berechnetes Molekulargewicht: 101 aa, 11 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC093965
• Gene symbol: PF4
• Gene ID (NCBI): 5196
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The chemokine (C-X-C motif) ligand 4 (CXCL4) is also named as PF4 (Platelet factor-4) and SCYB4. The platelets, the most abundant source of CXCL4 in vivo, control profibrotic macrophage activation via CXCL4. (PMID: 36807143). Exogenous CXCL4 drove profibrotic activation of monocyte-derived dendritic cells in vitro (PMID: 33042127). Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation.