CXCL4/PF4 Rekombinanter Antikörper

CXCL4/PF4 Rekombinant Antikörper für FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242741F10

Kat-Nr. : 98397-2-PBS

Synonyme

C-X-C motif chemokine 4, CXCL4, PF-4, Platelet factor 4

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  FC experiment of Transfected HEK-293T using 98397-2-RR (same clone as 98397-2-PBS)

  1x10^6 HEK-293T cells or CXCL4/PF4 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse PF4 Rabbit RecAb (98397-2-RR, Clone: 242741F10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98397-2-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98397-2-RR (same clone as 98397-2-PBS)

  1x10^6 HEK-293T cells (left) or CXCL4/PF4 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse PF4 Rabbit RecAb (98397-2-RR, Clone: 242741F10) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98397-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02069-2

  Cytometric bead array standard curve of MP02069-2, MOUSE CXCL4/PF4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98397-2-PBS. Detection antibody: 85738-2-PBS. Standard: Eg3345. Range: 0.781-50 ng/mL.

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Geprüfte Anwendungen

Produktinformation

98397-2-PBS bindet in FC (Intra), Cytometric bead array, Indirect ELISA CXCL4/PF4 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CXCL4/PF4 fusion protein Eg3345
• Vollständiger Name: platelet factor 4
• Berechnetes Molekulargewicht: 11kd
• GenBank-Zugangsnummer: NM_019932
• Gene symbol: Pf4
• Gene ID (NCBI): 56744
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CXCL4, also known as platelet factor 4 (PF-4), is the oldest member of the chemokine family. CXCL4 is a chemokine produced by activated platelets and immune cells involved in pathological conditions such as cancer, infections and inflammatory diseases like systemic sclerosis (SSc), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), among others. CXCL4 is present in the α-granules of platelets in amounts that constitute up to 2% platelet protein mass. CXCL4 plays a determinant role in distinct physiological processes such as in hematopoiesis, angiogenesis, coagulation and modulation of immune responses. For instance, CXCL4 can prevent monocyte apoptosis and promote cell survival, induces the production of TNF and reactive oxygen species (ROS) and promotes monocyte differentiation into a unique macrophage-like phenotype.