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PFAS Polyklonaler Antikörper

PFAS Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24716-1-PBS

Synonyme

EC:6.3.5.3, FGAM synthase, FGAMS, FGAR amidotransferase, FGARAT

Filter:
  • Western Blot (WB) analysis of various lysates using PFAS Polyclonal antibody (24716-1-AP)

    WB analysis using 24716-1-AP (same clone as 24716-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24716-1-AP (PFAS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24716-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PFAS Polyclonal antibody (24716-1-AP)

    WB analysis of HeLa using 24716-1-AP (same clone as 24716-1-PBS)

    WB result of PFAS antibody (24716-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFAS transfected HeLa cells. This data was developed using the same antibody clone with 24716-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PFAS Polyclonal antibody (24716-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 24716-1-AP (same clone as 24716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24716-1-AP (PFAS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24716-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PFAS Polyclonal antibody (24716-1-AP)

    IF Staining of A431 using 24716-1-AP (same clone as 24716-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PFAS antibody (24716-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24716-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

24716-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PFAS und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PFAS fusion protein Ag20162
Vollständiger Name phosphoribosylformylglycinamidine synthase
Berechnetes Molekulargewicht 1338 aa, 145 kDa
Beobachtetes Molekulargewicht140-150 kDa
GenBank-ZugangsnummerBC167158
Gene symbol PFAS
Gene ID (NCBI) 5198
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphoribosylformylglycinamidine synthase (PFAS) is a highly conserved enzyme that catalyzes the fourth step of de novo purine synthesis. It catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.