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PFKFB2 Rekombinanter Antikörper

PFKFB2 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243136E5

Kat-Nr. : 85639-6-PBS

Synonyme

6PF-2-K/Fru-2,6-P2ase 2, 6PF-2-K/Fru-2,6-P2ase heart-type isozyme, 6-phosphofructo-2-kinase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, Fructose-2,6-bisphosphatase

Filter:
  • Western Blot (WB) analysis of various lysates using PFKFB2 Recombinant antibody (85639-6-RR)

    WB analysis using 85639-6-RR (same clone as 85639-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85639-6-RR (PFKFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85639-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85639-6-RR

    Affinity and Kinetic Characterization of 85639-6-RR

    Biolayer interferometry (BLl) kinetic assays of 85639-6-RR against Human PFKFB2 were performed. The affinity constant is 2.74 nM.

Geprüfte Anwendungen

Produktinformation

85639-6-PBS bindet in WB, Indirect ELISA PFKFB2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PFKFB2 fusion protein Ag32347
Vollständiger Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Berechnetes Molekulargewicht 505 aa, 58 kDa
Beobachtetes Molekulargewicht 55 kDa
GenBank-ZugangsnummerBC075076
Gene symbol PFKFB2
Gene ID (NCBI) 5208
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PFKFB2(6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatase 2) is also named as PFK-2/FBPase-2, which is a bifunctional enzyme responsible for the synthesis and breakdown of Fru-2,6-P2 in the regulation of glycolysis(PMID:10095107). In the C-terminal section, it belongs to the phosphoglycerate mutase family. The enzyme is a dimer of identical subunits, each composed of 505 amino acids and containing three discrete domains(PMID: 2552438). It has 2 isoforms produced by alternative splicing.