PFKFB4 Rekombinanter Antikörper

PFKFB4 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242962H8

Kat-Nr. : 85444-3-PBS

Synonyme

242962H8, 6PF 2 K/Fru 2,6 P2ase 4, PFK/FBPase 4

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  WB analysis using 85444-3-RR (same clone as 85444-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85444-3-RR (PFKFB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85444-3-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01998-1

  Sandwich ELISA standard curve of MP01998-1, Human PFKFB4 Recombinant Matched Antibody Pair - PBS only. 85444-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30571. 85444-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-25 ng/mL

• 

  Sample test of MP01998-1

  The mean PFKFB4 concentration was determined to be 167.95 ng/mL in HepG2 cell extract based on a 1.8 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP01998-1

  Cytometric bead array standard curve of MP01998-1, PFKFB4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85444-3-PBS. Detection antibody: 85444-2-PBS. Standard: Ag30571. Range: 0.781-25 ng/mL.

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  Affinity and Kinetic Characterization of 85444-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85444-3-RR against Human PFKFB4 were performed. The affinity constant is 0.705 nM.

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Geprüfte Anwendungen

Produktinformation

85444-3-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test PFKFB4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PFKFB4 fusion protein Ag30571
• Vollständiger Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: NM_001317134
• Gene symbol: PFKFB4
• Gene ID (NCBI): 5210
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.