PFKM Polyklonaler Antikörper

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• KD/KO Validated

PFKM Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 30326-1-AP

Synonyme

PFK-A, PFK A, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, muscle type

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  WB analysis using 30326-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30326-1-AP (PFKM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 30326-1-AP

  WB result of PFKM antibody (30326-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFKM transfected HEK-293 cells.

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  WB analysis using 30326-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30326-1-AP (PFKM antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 30326-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30326-1-AP (PFKM antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 30326-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30326-1-AP (PFKM antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 30326-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed 48-well HepG2 cells using PFKM antibody (30326-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HEK-293-Zellen, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: 48-well HepG2 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

30326-1-AP bindet in WB, IHC, IF/ICC, ELISA PFKM und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PFKM fusion protein Ag30840
• Vollständiger Name: phosphofructokinase, muscle
• Beobachtetes Molekulargewicht: 75-85 kDa
• GenBank-Zugangsnummer: BC021203
• Gene symbol: PFKM
• Gene ID (NCBI): 5213
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and two domains subfamily. PFKM catalyzes the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular masses of 85, 82 and 93 kDa.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; Gboxin Induced Apoptosis and Ferroptosis of Cervical Cancer Cells by Promoting Autophagy-Mediated Inhibition of Nrf2 Signaling Under Low-Glucose Conditions; Authors - Wei Liu