PFKP Monoklonaler Antikörper

PFKP Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H4D4

Kat-Nr. : 68129-1-PBS

Synonyme

1H4D4, 6-phosphofructokinase type C, ATP-dependent 6-phosphofructokinase, platelet type, ATP-PFK, EC:2.7.1.11

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  WB analysis using 68129-1-Ig (same clone as 68129-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68129-1-Ig (PFKP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

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  WB analysis using 68129-1-Ig (same clone as 68129-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68129-1-Ig (PFKP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68129-1-Ig (same clone as 68129-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PFKP antibody (68129-1-Ig, Clone: 1H4D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 68129-1-Ig (same clone as 68129-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using PFKP antibody (68129-1-Ig, Clone: 1H4D4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68129-1-PBS bindet in WB, IF/ICC, Indirect ELISA PFKP und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PFKP fusion protein Ag25206
• Vollständiger Name: phosphofructokinase, platelet
• Berechnetes Molekulargewicht: 784 aa, 86 kDa
• Beobachtetes Molekulargewicht: 80-86 kDa
• GenBank-Zugangsnummer: BC029138
• Gene symbol: PFKP
• Gene ID (NCBI): 5214
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).