PFN2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PFN2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

2H7C12

Kat-Nr. : 60094-2-Ig

Synonyme

D3S1319E, PFL, PFN2, Prifilin 2, PROF2, profilin 2, Profilin II

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 60094-2-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60094-2-Ig (PFN2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 60094-2-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60094-2-Ig (PFN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 60094-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human lung cancer using 60094-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of mouse brain using 60094-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 60094-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 60094-2-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 60094-2-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Skelettmuskelgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

60094-2-Ig bindet in WB, IHC, IF, ELISA PFN2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PFN2 fusion protein Ag5043
• Vollständiger Name: profilin 2
• Berechnetes Molekulargewicht: 140 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC018049
• Gene symbol: PFN2
• Gene ID (NCBI): 5217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PFN2 (profilin 2) is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. The MW of this protein is 15 kDa, and this antibody specially recognises the 15 kDa protein.

Publikationen

Species	Application	Title
mouse	WB	J Cell Biol; NCAM regulates temporal specification of neural progenitor cells via profilin2 during corticogenesis.; Authors - Rui Huang ; KD Validated
human	WB,IHC	Int J Oncol; Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer.; Authors - Hui Zhang
mouse	WB	Front Mol Neurosci; Integrated proteomics and metabolomics analysis of D-pinitol function during hippocampal damage in streptozocin-induced aging-accelerated mice; Authors - Xiaoxia Li
human	WB,IHC	Mol Med; ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy.; Authors - Lihong Lu ; KD Validated
human	WB,IHC	J Mammary Gland Biol Neoplasia; Circ_0008500 Knockdown Improves Radiosensitivity and Inhibits Tumorigenesis in Breast Cancer Through the miR-758-3p/PFN2 Axis.; Authors - Deyou Kong
mouse	WB,IF	Biochem Biophys Res Commun; PFN2a, a new partner of RARα in the cytoplasm.; Authors - Dina Andriamoratsiresy