PFN2 Monoklonaler Antikörper

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• KD/KO Validated

PFN2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H7C12

Kat-Nr. : 60094-2-PBS

Synonyme

Prifilin 2, Profilin II, profilin 2, PFL, D3S1319E

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  WB analysis of mouse brain using 60094-2-Ig (same clone as 60094-2-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60094-2-Ig (PFN2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 60094-2-Ig (same clone as 60094-2-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60094-2-Ig (PFN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60094-2-Ig (same clone as 60094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60094-2-Ig (same clone as 60094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 60094-2-Ig (same clone as 60094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 60094-2-Ig (same clone as 60094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 60094-2-Ig (same clone as 60094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60094-2-Ig (same clone as 60094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60094-2-Ig (same clone as 60094-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PFN2 antibody (60094-2-Ig, Clone: 2H7C12 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60094-2-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PFN2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PFN2 fusion protein Ag5043
• Vollständiger Name: profilin 2
• Berechnetes Molekulargewicht: 140 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC018049
• Gene symbol: PFN2
• Gene ID (NCBI): 5217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PFN2 (profilin 2) is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. The MW of this protein is 15 kDa, and this antibody specially recognises the 15 kDa protein.