PGAM1 Monoklonaler Antikörper

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PGAM1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H2A9

Kat-Nr. : 67470-1-PBS

Synonyme

BPG dependent PGAM 1, PGAM B, PGAM1, PGAMA, Phosphoglycerate mutase 1

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  WB analysis using 67470-1-Ig (same clone as 67470-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67470-1-Ig (PGAM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

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  WB analysis using 67470-1-Ig (same clone as 67470-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67470-1-Ig (PGAM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67470-1-Ig (same clone as 67470-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67470-1-Ig (PGAM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67470-1-Ig (same clone as 67470-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67470-1-Ig (PGAM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67470-1-Ig (same clone as 67470-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGAM1 antibody (67470-1-Ig, Clone: 2H2A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67470-1-PBS bindet in WB, IHC, IF/ICC, ELISA PGAM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PGAM1 fusion protein Ag9250
• Vollständiger Name: phosphoglycerate mutase 1 (brain)
• Berechnetes Molekulargewicht: 254 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC011678
• Gene symbol: PGAM1
• Gene ID (NCBI): 5223
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PGAM1(phosphoglycerate mutase 1) is also named as PGAMA,PGAM-B and belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The homodimer PGAM1 is expressed mainly in liver, kidney, brain and overexpressed in a variety of human cancers, including breast carcinoma, colorectal cancer, lung cancer, prostate cancer, oral squamous cell carcinoma, esophageal squamous cell carcinomas and also associated with certain virus infection. PGAM1 could be developed as a useful diagnostic biomarker, as well as a potential therapeutic target for hepatocellular carcinoma (PMID:20403181). This antibody may also recognize PGAM2 and PGAM4 due to the high homology.