PGAM2 Polyklonaler Antikörper

PGAM2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15550-1-PBS

Synonyme

BPG dependent PGAM 2, BPG-dependent PGAM 2, EC:5.4.2.11, Muscle-specific phosphoglycerate mutase, PGAM M

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  WB analysis using 15550-1-AP (same clone as 15550-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 15550-1-AP (same clone as 15550-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 15550-1-AP (same clone as 15550-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 15550-1-AP (same clone as 15550-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15550-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 15550-1-AP (same clone as 15550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15550-1-AP (same clone as 15550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 15550-1-AP (same clone as 15550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 15550-1-AP (same clone as 15550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 15550-1-AP (same clone as 15550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 15550-1-AP (same clone as 15550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 15550-1-AP (same clone as 15550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15550-1-PBS bindet in WB, IHC, ELISA PGAM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PGAM2 fusion protein Ag7908
• Vollständiger Name: phosphoglycerate mutase 2 (muscle)
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC001904
• Gene symbol: PGAM2
• Gene ID (NCBI): 5224
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphoglycerate mutase (PGAM), an important enzyme in the glycolytic pathway, catalyzes the transfer of a phosphate group between the 2 and the 3 positions of glyceric acid. The muscle-specific isoform (type M, PGAM2) of phosphoglycerate mutase (PGAM) is a housekeeping enzyme and it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the glycolysis process to release energy. It is encoded by the Pgam2 gene. In addition, it is demonstrated that PGAM2 locates both in cytoplasm and nuclei, and takes part in the glycometabolism process of cytoplasm and nuclei(PMID: 18499067). Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). This antibody may also recognize PGAM1 and PGAM4 due to the high homology.