PGAM5 Polyklonaler Antikörper

PGAM5 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28445-1-PBS

Synonyme

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  WB analysis using 28445-1-AP (same clone as 28445-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28445-1-AP (PGAM5 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28445-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 28445-1-AP (same clone as 28445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 28445-1-AP (same clone as 28445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 28445-1-AP (same clone as 28445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 28445-1-AP (same clone as 28445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 28445-1-AP (same clone as 28445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 28445-1-AP (same clone as 28445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28445-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 28445-1-AP (same clone as 28445-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PGAM5 antibody (28445-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28445-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28445-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PGAM5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PGAM5 fusion protein Ag28195
• Vollständiger Name: phosphoglycerate mutase family member 5
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC008196
• Gene symbol: PGAM5
• Gene ID (NCBI): 192111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphoglycerate mutase 5 (PGAM5) is a mitochondrial Serine (Ser)/Threonine (Thr) phosphatase normally located in the inner mitochondrial membrane. Upon mitochondrial dysfunction, PGAM5 recruits and dephosphorylates Drp1 at Ser-637, triggers its GTPase activity and promotes mitochondrial fission. PGAM5 regulates mitophagy by stabilizing PINK1 under stress conditions, which recruits E3 ubiquitin ligase PARKIN for degradation of the damaged mitochondria. PGAM5 can be cleaved and released to the cytoplasm through PARKIN, which activates Wnt signaling and induces mitochondrial biogenesis (PMID: 32439975). PGAM5 has 2 isoforms with the molecular mass of 28 and 32 kDa.