PGAM5 Monoklonaler Antikörper

PGAM5 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

1C8B9

Kat-Nr. : 68116-1-Ig

Synonyme

1C8B9, Bcl-XL-binding protein v68, BXLBv68, EC:3.1.3.16, Serine/threonine-protein phosphatase PGAM5, mitochondrial

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  WB analysis using 68116-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68116-1-Ig (PGAM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of rabbit brain using 68116-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68116-1-Ig (PGAM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 68116-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68116-1-Ig (PGAM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 68116-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 68116-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 68116-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68116-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, Hausschwein-Hirngewebe, Kaninchenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

68116-1-Ig bindet in WB, IHC, IF, ELISA PGAM5 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PGAM5 fusion protein Ag28195
• Vollständiger Name: phosphoglycerate mutase family member 5
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC008196
• Gene symbol: PGAM5
• Gene ID (NCBI): 192111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphoglycerate mutase 5 (PGAM5) is a mitochondrial Serine (Ser)/Threonine (Thr) phosphatase normally located in the inner mitochondrial membrane. Upon mitochondrial dysfunction, PGAM5 recruits and dephosphorylates Drp1 at Ser-637, triggers its GTPase activity and promotes mitochondrial fission. PGAM5 regulates mitophagy by stabilizing PINK1 under stress conditions, which recruits E3 ubiquitin ligase PARKIN for degradation of the damaged mitochondria. PGAM5 can be cleaved and released to the cytoplasm through PARKIN, which activates Wnt signaling and induces mitochondrial biogenesis (PMID: 32439975). PGAM5 has 2 isoforms with the molecular mass of 28 and 32 kDa.

Publikationen

Species	Application	Title
rat	WB,IF	Biomed Pharmacother; Effects of dapagliflozin monotherapy and combined aerobic exercise on skeletal muscle mitochondrial quality control and insulin resistance in type 2 diabetes mellitus rats; Authors - Liangzhi Zhang
rat,mouse	WB	MedComm (2020); SIRT1 Alleviates Mitochondrial Fission and Necroptosis in Cerebral Ischemia/Reperfusion Injury via SIRT1-RIP1 Signaling Pathway; Authors - Xuan Wei
mouse	IF	CNS Neurosci Ther; Cleaving PINK1 or PGAM5? Involvement of PARL in Methamphetamine-Induced Excessive Mitophagy and Neuronal Necroptosis; Authors - Di An